High-frequency ultrasound-assisted drug delivery of chia, cress, and flax conjugated hematite iron oxide nanoparticle for sono-photodynamic lung cancer treatment in vitro and in vivo

Author:

Abd El-Kaream Samir Ali,Zedan Doha Farhat Mohamed,Mohamed Hagar Mohamed,Soliman Amal Saleh Mohamed,El-Kholey Sohier Mahmoud,Nasra Mohammed Kamal El-Dein

Abstract

Abstract Background Sono-photodynamic therapy (SPDT), which combines photodynamic (PDT) and sonodynamic (SDT) therapies with sensitizers, offers new avenues for cancer treatment. Even though new sensitizers for SPDT have been synthesized with great success, few of them are effectively used. The limited tumor-targeting specificity, inability to transport the sensitizers deeply intratumorally, and the deteriorating tumor microenvironment limit their anti-tumor effectiveness. The current study was carried out aiming at high-frequency ultrasound-assisted drug delivery of chia, cress and flax conjugated hematite iron oxide nanoparticles (CCF–HIONP) for photothermal–photodynamic lung cancer (LCA) treatment in vitro and in vivo as activated cancer treatment up-to-date modality. Materials and methods The study was conducted in vitro on human LCA cells (A-549) and the study protocol application groups in vivo on Swiss albino mice treated with benzo[a]pyrene only and were not received any treatment for inducing LCA, and only after LCA induction the study treatment protocol began, treatment was daily with CCF–HIONP as HIFU–SPDT sensitizer with or without exposure to laser (IRL) or high-frequency ultrasound (HIFU–US) or a combination of laser and/or high-frequency ultrasound for 3 min for 2 weeks. Results Revealed that HIONP can be employed as effective CCF delivery system that directly targets LCA cells. In addition, CCF–HIONP is a promising HIFU–SPS for HIFU–SPDT and when combined with HIFU–SPDT can be very effective in treatment of LCA–A549 in vitro (cell viability decreased in a dose-dependent basis, the cell cycle progression in G0/G1 was slowed down, and cell death was induced as evidenced by an increase in the population of Pre-G cells, an increase in early and late apoptosis and necrosis, and an increase in autophagic cell death) and benzo[a]pyrene LCA-induce mice in vivo (decreased oxidative stress (MDA), and ameliorated enzymatic and non-enzymatic antioxidants (SOD, GR, GPx, GST, CAT, GSH, and TAC) as well as renal (urea, creatinine) and hepatic (ALT, AST) functions, induced antiproliferative genes (caspase 3,9, p53, Bax, TNFalpha), suppressed antiapoptotic and antiangiogenic genes (Bcl2,VEGF respectively) and effectively reducing the growth of tumors and even leading to cancer cell death. This process could be attributed to photochemical and/or high-frequency sono-chemical activation mechanism HIFU–SPDT. Conclusions The results indicate that CCF–HIONP has great promise as an innovative, effective delivery system for selective localized treatment of lung cancer that is activated by HIFU–SPDT.

Funder

Alexandria University

Publisher

Springer Science and Business Media LLC

Reference69 articles.

1. Abd El-Kaream SA (2019) Biochemical and biophysical study of chemopreventive and chemotherapeutic anti-tumor potential of some Egyptian plant extracts. Biochem Biophys Rep 18:100637

2. Abd El-Kaream SA, Abd Elsamie GH, Abd-Alkareem AS (2018) Sono-photodynamic modality for cancer treatment using bio-degradable bio-conjugated sonnelux nanocomposite in tumor-bearing mice: activated cancer therapy using light and ultrasound. Biochem Biophys Res Commun 503:1075

3. Abd El-Kaream SA, Abd Elsamie GH, Abbas AJ (2019a) Sono and photo sensitized gallium-porphyrin nanocomposite in tumor-bearing mice: new concept of cancer treatment. Am J Nanotechnol Nanomed 2:5

4. Abd El-Kaream SA, Abd Elsamie GH, Metwally MA, Almamoori AYK (2019b) Sono and photo stimulated chlorine e6 nanocomposite in tumor-bearing mice: upcoming cancer treatment. Radiol Med Diag Imag 2:1

5. Abd El-Kaream SA, Abd Elsamie GH, Isewid GA (2019c) Laser and ultrasound activated photolon nanocomposite in tumor-bearing mice: new cancer fighting drug-technique. Arch Oncol Cancer Ther 2:1

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