Exploring motion using geometric morphometrics in microscopic aquatic invertebrates: ‘modes’ and movement patterns during feeding in a bdelloid rotifer model species

Abstract

Abstract Background Movement is a defining aspect of animals, but it is rarely studied using quantitative methods in microscopic invertebrates. Bdelloid rotifers are a cosmopolitan class of aquatic invertebrates of great scientific interest because of their ability to survive in very harsh environment and also because they represent a rare example of an ancient lineage that only includes asexually reproducing species. In this class, Adineta ricciae has become a model species as it is unusually easy to culture. Yet, relatively little is known of its ethology and almost nothing on how it behaves during feeding. Methods To explore feeding behaviour in A. ricciae, as well as to provide an example of application of computational ethology in a microscopic invertebrate, we apply Procrustes motion analysis in combination with ordination and clustering methods to a laboratory bred sample of individuals recorded during feeding. Results We demonstrate that movement during feeding can be accurately described in a simple two-dimensional shape space with three main ‘modes’ of motion. Foot telescoping, with the body kept straight, is the most frequent ‘mode’, but it is accompanied by periodic rotations of the foot together with bending while the foot is mostly retracted. Conclusions Procrustes motion analysis is a relatively simple but effective tool for describing motion during feeding in A. ricciae. The application of this method generates quantitative data that could be analysed in relation to genetic and ecological differences in a variety of experimental settings. The study provides an example that is easy to replicate in other invertebrates, including other microscopic animals whose behavioural ecology is often poorly known.