Involvement of mechano-sensitive Piezo1 channel in the differentiation of brown adipocytes

Author:

Kenmochi Manato,Kawarasaki Satoko,Takizawa Satsuki,Okamura Kazuhiko,Goto Tsuyoshi,Uchida Kunitoshi

Abstract

AbstractBrown adipocytes expend energy via heat production and are a potential target for the prevention of obesity and related metabolic disorders. Piezo1 is a Ca2+-permeable non-selective cation channel activated by mechanical stimuli. Piezo1 is reported to be involved in mechano-sensation in non-sensory tissues. However, the expression and roles of Piezo1 in brown adipocytes have not been well clarified. Here, we generated a brown adipocyte line derived from UCP1-mRFP1 transgenic mice and showed that Piezo1 is expressed in pre-adipocytes. Application of Yoda-1, a Piezo1 agonist, suppressed brown adipocyte differentiation, and this suppression was significantly attenuated by treatment with a Piezo1 antagonist and by Piezo1 knockdown. Furthermore, the suppression of brown adipocyte differentiation by Yoda-1 was abolished by co-treatment with a calcineurin inhibitor. Thus, these results suggest that activation of Piezo1 suppresses brown adipocyte differentiation via the calcineurin pathway.

Funder

Japan Society for the Promotion of Science

Takeda Science Foundation

Publisher

Springer Science and Business Media LLC

Subject

Physiology

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