High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
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Published:2009-01-09
Issue:1
Volume:10
Page:
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ISSN:1471-2172
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Container-title:BMC Immunology
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language:en
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Short-container-title:BMC Immunol
Author:
Mahshid Yilmaz,Lisy Marcus-René,Wang Xiao,Spanbroek Rainer,Flygare Jenny,Christensson Birger,Björkholm Magnus,Sander Birgitta,Habenicht Andreas JR,Claesson Hans-Erik
Abstract
Abstract
Background
Human B lymphocytes can produce leukotriene B4 but the biological function of the 5-lipoxygenase (5-LO) pathway in B cells is unclear. In order to better understand and define the role of 5-LO in B cells, we investigated the expression of 5-LO mRNA and protein in subsets of B cells from human tonsils and different types of B cell lymphoma.
Results
Based on RT-PCR and western blot/immunohistochemical staining, with a polyclonal antibody raised against 5-LO, high expression of 5-LO was found in mantle zone B cells from tonsils. By contrast, only a weak expression of 5-LO was detected in germinal centre cells and no expression in plasma cells from tonsils. This pattern of 5-LO expression was preserved in malignant lymphoma with high expression in mantle B cell lymphoma (MCL) and weak or no expression in follicular lymphoma. Primary leukemized MCL, so called B-prolymphocytic leukaemia cells, and MCL cell lines also expressed 5-LO and readily produced LTB4 after activation.
Conclusion
The present report demonstrates the expression of 5-LO mainly in normal and malignant mantle zone B cells while the expression is low or absent in germinal centre B cells and plasma cells, indicating a role of the 5-LO pathway in B cells before the cells finally differentiate to plasma cells.
Publisher
Springer Science and Business Media LLC
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