Author:
Victor Jefferson R,Muniz Bruno P,Fusaro Ana E,de Brito Cyro A,Taniguchi Eliana F,Duarte Alberto JS,Sato Maria N
Abstract
Abstract
Background
Preconception allergen immunization prevents neonatal allergen sensitization in mice by a complex interaction between regulatory cells/factors and antibodies. The present study assessed the influence of maternal immunization with ovalbumin (OVA) on the immune response of 3 day-old and 3 week-old offspring immunized or non-immunized with OVA and evaluated the effect of IgG treatment during fetal development or neonatal period.
Results
Maternal immunization with OVA showed increased levels of FcγRIIb expression in splenic B cells of neonates, which were maintained for up to 3 weeks and not affected by additional postnatal OVA immunization. Maternal immunization also exerted a down-modulatory effect on both IL-4 and IFN-γ-secreting T cells and IL-4 and IL-12- secreting B cells. Furthermore, immunized neonates from immunized mothers showed a marked inhibition of antigen-specifc IgE Ab production and lowered Th2/Th1 cytokine levels, whereas displaying enhanced FcγRIIb expression on B cells. These offspring also showed reduced antigen-specific proliferative response and lowered B cell responsiveness. Moreover, in vitro evaluation revealed an impairment of B cell activation upon engagement of B cell antigen receptor by IgG from OVA-immunized mice. Finally, in vivo IgG transference during pregnancy or breastfeeding revealed that maternal Ab transference was able to increase regulatory cytokines, such as IL-10, in the prenatal stage; yet only the postnatal treatment prevented neonatal sensitization. None of the IgG treatments induced immunological changes in the offspring, as it was observed for those from OVA-immunized mothers.
Conclusion
Maternal immunization upregulates the inhibitory FcγRIIb expression on offspring B cells, avoiding skewed Th2 response and development of allergy. These findings contribute to the advancement of prophylactic strategies to prevent allergic diseases in early life.
Publisher
Springer Science and Business Media LLC
Reference37 articles.
1. Jarrett E, Hall E: Selective Suppression of IgE Antibody Responsiveness by Maternal Influence. Nature. 1979, 280: 145-147. 10.1038/280145a0.
2. Jarrett EE, Hall E: IgE Suppression by Maternal IgG. Immunology. 1983, 48: 49-58.
3. Jarrett EE, Hall E: The Development of IgE-Suppressive Immunocompetence in Young Animals - Influence of Exposure to Antigen in the Presence or Absence of Maternal Immunity. Immunology. 1984, 53: 365-373.
4. Roberts SA, Turner MW: Specific Suppression of Rat Ige Responses with Milk from Immunized Females and with Feeds of Serum Antibody. Immunology. 1983, 48: 195-199.
5. Seeger M, Thierse HJ, Lange H, Shaw L, Hansen H, Lemke H: Antigen-independent suppression of the IgE immune response to bee venom phospholipase A(2) by maternally derived monoclonal IgG antibodies. Eur J Immunol. 1998, 28: 2124-2130. 10.1002/(SICI)1521-4141(199807)28:07<2124::AID-IMMU2124>3.0.CO;2-A.
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