Author:
Ouyang Long-Ling,Chen Si-Hong,Li Yan,Zhou Zhi-Gang
Abstract
Abstract
Background
Arachidonic acid (ArA) is important for human health because it is one of the major components of mammalian brain membrane phospholipids. The interest in ArA inspired the search for a new sustainable source, and the green microalga Myrmecia incisa Reisigl H4301 has been found a potential ArA-producer due to a high content of intracellular ArA. To gain more molecular information about metabolism pathways, including the biosynthesis of ArA in the non-model microalga, a transcriptomic analysis was performed.
Results
The 454 pyrosequencing generated 371,740 high-quality reads, which were assembled into 51,908 unique sequences consisting of 22,749 contigs and 29,159 singletons. A total of 11,873 unique sequences were annotated through BLAST analysis, and 3,733 were assigned to Gene Ontology (GO) categories. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis uncovered a C4-like photosynthesis pathway in M. incisa. The biosynthesis pathways of lipid particularly those of ArA and triacylglycerol (TAG) were analyzed in detail, and TAG was proposed to be accumulated in oil bodies in the cytosol with the help of caleosin or oil globule-associated proteins. In addition, the carotenoid biosynthesis pathways are discussed.
Conclusion
This transcriptomic analysis of M. incisa enabled a global understanding of mechanisms involved in photosynthesis, de novo biosynthesis of ArA, metabolism of carotenoids, and accumulation of TAG in M. incisa. These findings provided a molecular basis for the research and possibly economic exploitation of this ArA-rich microalga.
Publisher
Springer Science and Business Media LLC
Reference72 articles.
1. Crawford MA, Sinclair AJ: Nutritional influences in the evolution of mammalian brain. Lipids, malnutrition and the developing brain. Edited by: Elliott K, Knight J. 1972, London: Associated Scientific Publishers, 267-292.
2. Lee JY, Hwang DH: Dietary fatty acids and eicosanoids. Fatty Acids in Foods and Their Health Implications. Edited by: Chow CK. 2008, Boca Raton: CRC press, 713-726. 3
3. Innis SM: Essential fatty acids in growth and development. Prog Lipid Res. 1991, 30 (1): 39-103. 10.1016/0163-7827(91)90006-Q.
4. Carlson SE, Werkman SH, Peeples JM, Cooke RJ, Tolley EA: Arachidonic acid status correlates with first year growth in preterm infants. Proc Natl Acad Sci U S A. 1993, 90 (3): 1073-1077. 10.1073/pnas.90.3.1073.
5. Crawford MA, Costeloe K, Ghebremeskel K, Phylactos A, Skirvin L, Stacey F: Are deficits of arachidonic and docosahexaenoic acids responsible for the neural and vascular complications of preterm babies?. Am J Clin Nutr. 1997, 66 (Suppl 4): 1032S-1041S.
Cited by
24 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献