Author:
Qu Hongke,Fan Chunmei,Chen Mingjian,Zhang Xiangyan,Yan Qijia,Wang Yumin,Zhang Shanshan,Gong Zhaojian,Shi Lei,Li Xiayu,Liao Qianjin,Xiang Bo,Zhou Ming,Guo Can,Li Guiyuan,Zeng Zhaoyang,Wu Xu,Xiong Wei
Abstract
AbstractThe cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods.
Graphical Abstract
Funder
National Natural Science Foundation of China
Overseas Expertise Introduction Project for Discipline Innovation
Natural Science Foundation of Hunan Province
the Fundamental Research Funds for the Central Universities of Central South University
Publisher
Springer Science and Business Media LLC
Subject
Pharmaceutical Science,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,Medicine (miscellaneous),Bioengineering
Cited by
23 articles.
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