Abstract
Abstract
Background
The appearance of resistance against new treatments and the fact that HIV-1 can infect various cell types and develop reservoirs and sanctuaries makes it necessary to develop new therapeutic approaches to overcome those failures.
Results
Studies of cytotoxicity, genotoxicity, complexes formation, stability, resistance, release and particle size distribution confirmed that G2-SN15-PEG, G3-SN31-PEG, G2-SN15-PEG-FITC and G3-SN31-PEG-FITC dendrimers can form complexes with miRNAs being biocompatible, stable and conferring protection to these nucleic acids. Confocal microscopy and flow cytometry showed effective delivery of these four dendrimers into the target cells, confirming their applicability as delivery systems. Dendriplexes formed with the dendrimers and miRNAs significantly inhibited HIV-1 infection in PBMCs.
Conclusions
These dendrimers are efficient delivery systems for miRNAs and they specifically and significantly improved the anti-R5-HIV-1 activity of these RNA molecules.
Graphic Abstract
Funder
Instituto de Salud Carlos III
Publisher
Springer Science and Business Media LLC
Subject
Pharmaceutical Science,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,Medicine (miscellaneous),Bioengineering
Reference52 articles.
1. Bissel SJ, Wiley CA. Human immunodeficiency virus infection of the brain: pitfalls in evaluating infected/affected cell populations. Brain Pathol. 2004;14(1):97–108.
2. Zayyad Z, Spudich S. Neuropathogenesis of HIV: from initial neuroinvasion to HIV-associated neurocognitive disorder (HAND). Curr HIV/AIDS Rep. 2015;12(1):16–24.
3. A I. Guidelines for the use of antiretroviral agents in adults and adolescents living with HIV. . Department of Health and Human Services; 2019 [updated 10 July 2019]. https://aidsinfo.nih.gov/guidelines/html/1/adult-and-adolescent-arv/0.
4. EAC S. Guidelines for the clinical management and treatment of HIV-infected adults in Europe. 2018. https://www.eacsociety.org/guidelines/.
5. Diallo M, Adekpedjou R, Ahouada C, Ngangue P, Ly BA. Impact of pre-antiretroviral therapy CD4 counts on drug resistance and treatment failure: a systematic review. AIDS Rev. 2020;22(2):78–92.
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