Author:
Liu Jiayu,Wang Cenzhu,Jiang Ying,Zhou Yunxu,Chen Lingyan,Qian Zhiwen,Liu Lu,Wu Danping,Zhang Yan
Abstract
AbstractThe members of the classic B7 family regulate the immune microenvironment of several malignant tumors. However, the potential relationship between the B7 family and the breast cancer (BrCa) tumor immune microenvironment has remained elusive. In the present study, we provide a comprehensive explanation of the expression, clinical significance, mutation, and immune cell infiltration of B7 family molecules in BrCa. First, we recruited 10 patients with BrCa surgery from the Wuxi Maternal and Child Health Hospital and performed single-cell RNA sequencing (scRNA-seq) analysis to investigate the distribution of B7 family members in multiple immune cell subsets. We focused on B7-2, B7-H3, and B7-H5 molecules of the B7 family and constructed tumor microarrays by self-recruiting patients to perform multiple immunohistochemical (mIHC) analyses and study tumor expression of B7-2, B7-H3, B7-H5 and CD8+ immune cell infiltration. B7-H5 displayed a strong correlation with CD8+ immune cell infiltration. In summary, B7-H5 provides a new perspective for the identification of immunothermal subtypes of BrCa and could function as a switch to reverse BrCa from an “immunologically cold” state to an “immunologically hot” state.
Graphical abstract
Funder
Graduate Research and Innovation Projects of Jiangsu Province
General Program of Wuxi Medical Center of Nanjing Medical University
Doctoral Talent Fund of the Affiliated Wuxi People’s Hospital of Nanjing Medical University
Wuxi Science and Technology Bureau
Wuxi Double-Hundred Talent Fund Project
Wuxi Health Commission Precision Medicine Project
Jiangsu Provincial Maternal and Child Health Research Project
Jiangsu Provincial Six Talent Peaks Project
Publisher
Springer Science and Business Media LLC