Author:
Zhao Xuan,Chen Guanxuan,Wu Yawen,Li Xiao,Zhang Zhe,Xie Li,Song Xianrang,Song Xingguo
Abstract
Abstract
Purpose
The alterations of RNA profile in tumor-educated platelets (TEPs) have been described as a novel biosource for cancer diagnostics. This study aimed to explore the potential snoRNAs in TEP as biomarkers for diagnostics of hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC).
Methods
Platelets were isolated using low-speed centrifugation and subjected to a quantitative polymerase chain reaction (qPCR) for snoRNAs detection.
Results
Down-regulated SNORD12B and SNORD14E as well as up-regulated SNORA63 were identified in TEP from HBV-related HCC, which could act as diagnostic biomarkers for HBV-related HCC as well as the early disease. Besides, TEP SNORD12B, SNORD14E, and SNORA63 facilitate the diagnostic performance of AFP and achieve favorable diagnostics efficiency for HBV-related HCC when combined with platelet parameters.
Conclusions
Aberrant expression of SNORD12B, SNORA63, and SNORD14E in TEPs could serve as the novel and non-invasive biomarkers for HBV-related HCC diagnosis.
Funder
National Natural Science Foundation of China
Shandong Traditional Chinese Medicine Technology Project
Shandong Natural Science Foundation Innovation and Development Joint Fund
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Genetics,Oncology
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