Author:
Zhou Huakang,Khan Dilaware,Hussain Sajid Muhammad,Gerdes Norbert,Hagenbeck Carsten,Rana Majeed,Cornelius Jan Frederick,Muhammad Sajjad
Abstract
Abstract
Background
Smoking, alcohol abuse, and hypertension are – among others, potential risk factors for cardiovascular diseases. These risk factors generate oxidative stress and cause oxidative stress-induced DNA damage, resulting in cellular senescence and senescence-associated secretory phenotype (SASP). The SASP factors in feed-forward response exacerbate inflammation and cause tissue remodeling, resulting in atherosclerotic plaque formation and rupture.
Results
Colchicine inhibited ROS generation and mitigated oxidative stress-induced DNA damage. It dampened oxidative stress-induced endothelial cell senescence and improved the expression of DNA repair protein KU80 and aging marker Lamin B1. The drug attenuated the expression of senescence marker P21 at mRNA and protein levels. The pathway analysis showed that colchicine inhibited NF-κB and MAPKs pathways and subdued mTOR activation. Colchicine also attenuated mRNA expression of interleukin (IL)-1β, IL-6, IL-8, MCP-1, ICAM-1, and E-selectin. Furthermore, colchicine reduced the mRNA and protein expression of matrix metalloproteinase (MMP-2).
Conclusion
In summary, colchicine blocked oxidative stress-induced senescence and SASP by inhibiting the activation of NF-κB and MAPKs pathways.
Graphical Abstract
Funder
Universitätsklinikum Düsseldorf. Anstalt öffentlichen Rechts
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Clinical Biochemistry
Cited by
1 articles.
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