Abstract
AbstractThe efficient asymmetric bio-synthesis of chiral β-hydroxy esters is of great importance for industrial production. In this work, a simple and productive engineered E.coli cell-immobilized strategy was applied for the asymmetric reduction of MAA to (R)-HBME with high enantioselectivity. Compared with the corresponding inactivated free cells, the alginate-immobilized cells remained 45% of initial activity at 50 ℃ and 65% after reuse of 10 times. After 60 days of storage at 4 ℃, the immobilized cells maintained more than 80% relative activity. Immobilization contributed significantly to the improvement of thermal stability, pH tolerance, storage stability and operation stability without affecting the yield of product. The immobilized recombinant E. coli cell had absolute enantioselectivity for the asymmetric reduction of MAA to (R)-HBME with e.e. > 99.9%. Therefore, microbial cell immobilization is a perspective approach in asymmetric synthesis of chiral β-hydroxy esters for industrial applications.
Graphical Abstract
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
Renewable Energy, Sustainability and the Environment,Biomedical Engineering,Food Science,Biotechnology
Reference23 articles.
1. Al-Hasawi ZM, Abdel-Hamid MI, Almutairi AW, Touliabah HE (2020) Response of Pseudokirchneriella subcapitata in free and alginate immobilized cells to heavy metals toxicity. Molecules 25(12):2847. https://doi.org/10.3390/molecules25122847
2. Bickerstaff GF (2006) Immobilization of enzymes and cells. Met Mol Biol 25:232–232. https://doi.org/10.1385/0-89603-386-4:1
3. Carvalho C (2011) Enzymatic and whole cell catalysis: finding new strategies for old processes. Biotechnol Adv 29(1):75–83. https://doi.org/10.1016/j.biotechadv.2010.09.001
4. Chen XH (2013) Asymmetric synthesis of chiral alcohols catalyzed by Acetobacter sp. CCTCC M209061 and characterization of the anti-Prelog carbonyl reductase. South China University of Technology, Guangzhou
5. Chen XH, Wang XT, Lou WY, Li Y, Wu H, Zong MH, Smith TJ, Chen XD (2012) Immobilization of Acetobacter sp. CCTCC M209061 for efficient asymmetric reduction of ketones and biocatalyst recycling. Microb Cell Fact 11:119–132. https://doi.org/10.1186/1475-2859-11-119
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