Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris

Author:

Cai Peng,Li Yunxia,Zhai Xiaoxin,Yao Lun,Ma Xiaojun,Jia Lingyun,Zhou Yongjin J.ORCID

Abstract

Abstractα-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals. Graphical Abstract

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

DICP innovation grant

Publisher

Springer Science and Business Media LLC

Subject

Renewable Energy, Sustainability and the Environment,Biomedical Engineering,Food Science,Biotechnology

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