Structure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate
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Published:2020-07-09
Issue:1
Volume:7
Page:
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ISSN:2197-4365
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Container-title:Bioresources and Bioprocessing
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language:en
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Short-container-title:Bioresour. Bioprocess.
Author:
Dong Qilei, Yuan Shuguang, Wu Lian, Su Lingqia, Zhao Qiaoling, Wu Jing, Huang Weixue, Zhou JiahaiORCID
Abstract
AbstractCutinases could degrade insoluble polyester, including natural cutin and synthetic plastic. However, their turnover efficiency for polyester remains too low for industrial application. Herein, we report the 1.54-Å resolution X-ray crystal structure of a cutinase from Thermobifida fusca and modeling structure in complex with a cutin mimic oligo-polyester C24H42O8. These efforts subsequently guided our design of cutinase variants with less bulky residues in the vicinity of the substrate binding site. The L90A and I213A variants exhibit increased hydrolysis activity (5- and 2.4-fold, respectively) toward cutin and also showed enhanced cotton scouring efficiency compared with the wild-type enzyme.
Funder
Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences Program of Shanghai Academic Research Leader National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
Renewable Energy, Sustainability and the Environment,Biomedical Engineering,Food Science,Biotechnology
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