A comprehensive approach to stool donor screening for faecal microbiota transplantation in China
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Published:2021-11-27
Issue:1
Volume:20
Page:
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ISSN:1475-2859
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Container-title:Microbial Cell Factories
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language:en
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Short-container-title:Microb Cell Fact
Author:
He Jianquan, He Xingxiang, Ma Yonghui, Yang Luxi, Fang Haiming, Shang Shu, Xia Huping, Lian Guanghui, Tang Hailing, Wang Qizhi, Wang Junping, Lin Zhihui, Wen Jianbo, Liu Yuedong, Zhai Chunbao, Wang Wen, Jiang Xueliang, Xuan Ji, Liu Morong, Lu Shiyun, Li Xuejun, Wang Han, Ouyang Cong, Cao Man, Lin Aiqiang, Zhang Bangzhou, Wu Depei, Chen Ye, Xiao ChuanxingORCID
Abstract
Abstract
Background
Faecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridium difficile infections and chronic gastrointestional infections. However, the risks of FMT and the selection process of suitable donors remain insufficiently characterized. The eligibility rate for screening, underlying microbial basis, and core ethical issues of stool donors for FMT are yet to be elucidated in China.
Results
The potential stool donors were screened from December 2017 to December 2019 with the help of an online survey, clinical assessments, and stool and blood testing. Bioinformatics analyses were performed, and the composition and stability of gut microbiota in stool obtained from eligible donors were dynamically observed using metagenomics. Meanwhile, we build a donor microbial evaluation index (DoMEI) for stool donor screening. In the screening process, we also focused on ethical principles and requirements. Of the 2071 participants, 66 donors were selected via the screening process (3.19% success rate). Although there were significant differences in gut microbiota among donors, we found that the changes in the gut microbiota of the same donor were typically more stable than those between donors over time.
Conclusions
DoMEI provides a potential reference index for regular stool donor re-evaluation. In this retrospective study, we summarised the donor recruitment and screening procedure ensuring the safety and tolerability for FMT in China. Based on the latest advances in this field, we carried out rigorous recommendation and method which can assist stool bank and clinicians to screen eligible stool donor for FMT.
Funder
national natural science foundation of china national major science and technology projects of china national office for philosophy and social sciences
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Reference62 articles.
1. Ng SC, Kamm MA, Yeoh YK, Chan PKS, Zuo T, Tang W, Sood A, Andoh A, Ohmiya N, Zhou Y, et al. Scientific frontiers in faecal microbiota transplantation: joint document of Asia-Pacific Association of Gastroenterology (APAGE) and Asia-Pacific Society for Digestive Endoscopy (APSDE). Gut. 2020;69:83–91. 2. Haifer C, Kelly CR, Paramsothy S, Andresen D, Papanicolas LE, McKew GL, Borody TJ, Kamm M, Costello SP, Andrews JM, et al. Australian consensus statements for the regulation, production and use of faecal microbiota transplantation in clinical practice. Gut. 2020;69:801–10. 3. Núñez FP, Quera R, Bay C, Thomson P. Fecal microbiota transplant, its usefulness beyond Clostridioides difficile in gastrointestinal diseases. Gastroenterol Hepatol. 2021. https://doi.org/10.1016/j.gastrohep.2021.05.009. 4. Kassam Z, Dubois N, Ramakrishna B, Ling K, Qazi T, Smith M, Kelly CR, Fischer M, Allegretti JR, Budree S, et al. Donor screening for fecal microbiota transplantation. N Engl J Med. 2019;381:2070–2. 5. Tariq R, Weatherly R, Kammer P, Pardi DS, Khanna S. Donor screening experience for fecal microbiota transplantation in patients with recurrent C. difficile infection. J Clin Gastroenterol. 2018;52:146–50.
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