Author:
Zhang Furui,Ni Linhan,Zhang Zhen,Luo Xuegang,Wang Xuequan,Zhou Wenmiao,Chen Jiale,Liu Jing,Qu Yuliang,Liu Kunmei,Guo Le
Abstract
Abstract
Background
Helicobacter pylori (H. pylori) causes chronic gastric disease. An efficient oral vaccine would be mucosa-targeted and offer defense against colonization of invasive infection in the digestive system. Proteolytic enzymes and acidic environment in the gastrointestinal tract (GT) can, however, reduce the effectiveness of oral vaccinations. For the creation of an edible vaccine, L. lactis has been proposed as a means of delivering vaccine antigens.
Results
We developed a plSAM (pNZ8148-SAM) that expresses a multiepitope vaccine antigen SAM-WAE containing Urease, HpaA, HSP60, and NAP extracellularly (named LL-plSAM-WAE) to increase the efficacy of oral vaccinations. We then investigated the immunogenicity of LL-plSAM-WAE in Balb/c mice. Mice that received LL-plSAM-WAE or SAM-WAE with adjuvant showed increased levels of antibodies against H. pylori, including IgG and sIgA, and resulted in significant reductions in H. pylori colonization. Furthermore, we show that SAM-WAE and LL-plSAM-WAE improved the capacity to target the vaccine to M cells.
Conclusions
These findings suggest that recombinant L. lactis could be a promising oral mucosa vaccination for preventing H. pylori infection.
Funder
Natural Science Foundation of Ningxia Province,China
National Natural Science Foundation of China
Key R & D Plan Project of Ningxia Autonomous Region
Natural Science Foundation of Ningxia Province
Science Research Project of Ningxia’s Colleges
Ningxia Youth Top Talent Training Project
Publisher
Springer Science and Business Media LLC