Author:
Mori Kotaro,Fukui Kaho,Amatsu Ryotaro,Ishikawa Shu,Verrone Valeria,Wipat Anil,Meijer Wilfried J. J.,Yoshida Ken-ichi
Abstract
Abstract
Background
Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis.
Results
In this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation.
Conclusions
This transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus.
Funder
Royal Society
Spanish Government
Japan Society for the Promotion of Science
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Cited by
2 articles.
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