Author:
Sun Manman,Gao Alex Xiong,Liu Xiuxia,Yang Yankun,Ledesma-Amaro Rodrigo,Bai Zhonghu
Abstract
AbstractIn the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions.
Funder
Postgraduate Research & Practice Innovation Program of Jiangsu Province
National Natural Science Foundation of China
the 111 Project
Fundamental Research Funds for the Central Universities
British Council
Biotechnology and Biological Sciences Research Council
National First-Class Discipline Program of Light Industry Technology and Engineering
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Cited by
4 articles.
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