Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region-Reference-Cited by-同舟云学术

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Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

Published:2018-10-25 Issue:1 Volume:17 Page:
ISSN:1475-2859
Container-title:Microbial Cell Factories
language:en
Short-container-title:Microb Cell Fact

Author:

Liu Lin,Yu Haoran,Du Kun,Wang Zhiyan,Gan Yiru,Huang He

Funder

National Science and Technology Major Projects of China

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://link.springer.com/content/pdf/10.1186/s12934-018-1012-x.pdf

Reference47 articles.

1. Rascon A, Seidl DS, Jaffe WG, Aizman A. Inhibition of trypsins and chymotrypsins from different animal species: a comparative study. Comp Biochem Physiol B Biochem Mol Biol. 1985;82:375–8.

2. Yao L, Man C, Zhao F, Qu X, Han X, Sun D, Jiang Y. Expression of bovine trypsin in Lactococcus lactis. Int Dairy J. 2010;20:806–9.

3. Leiros HKS, Brandsdal BO, Andersen OA, Os V, Leiros I, Helland R, Otlewski J, Willassen NP, Smalas AO. Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements. Protein Sci. 2004;13:1056–70.

4. Finehout EJ, Cantor JR, Lee KH. Kinetic characterization of sequencing grade modified trypsin. Proteomics. 2005;5:2319–21.

5. Guo C, Liu Y, Yu HR, Du K, Gan YR, Huang H. A novel strategy for thermostability improvement of trypsin based on N-glycosylation within the omega-loop region. J Microbiol Biotechnol. 2016;26:1163–72.

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