Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process
Author:
Funder
South Asian University
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
http://link.springer.com/content/pdf/10.1186/s12934-019-1053-9.pdf
Reference46 articles.
1. Yang X, Hu W, Li F, Xia H, Zhang Z. Gene cloning, bacterial expression, in vitro refolding, and characterization of a single-chain Fv antibody against PreS1(21-47) fragment of HBsAg. Protein Expr Purif. 2005;41:341–8.
2. Rudolph R, Lilie H. In vitro folding of inclusion body proteins. FASEB J Off Publ Fed Am Soc Exp Biol. 1996;10:49–56.
3. Burgess RR. Refolding solubilized inclusion body proteins. Methods Enzymol. 2009;463:259–82.
4. Carrió MM, Villaverde A. Localization of chaperones DnaK and GroEL in bacterial inclusion bodies. J Bacteriol. 2005;187:3599–601.
5. Clark ED. Protein refolding for industrial processes. Curr Opin Biotechnol. 2001;12:202–7.
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