Fecal microbiota transplantation attenuates Escherichia coli infected outgrowth by modulating the intestinal microbiome
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Published:2023-02-17
Issue:1
Volume:22
Page:
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ISSN:1475-2859
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Container-title:Microbial Cell Factories
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language:en
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Short-container-title:Microb Cell Fact
Author:
Wang Yaping,He Yuanyuan,Liang Ying,Liu Han,Chen Xiushuang,Kulyar Muhammad Fakhar-e-Alam,Shahzad Asim,Wei Kunhua,Li Kun
Abstract
Abstract
Background
Given the crucial role of gut microbiota in animal and human health, studies on modulating the intestinal microbiome for therapeutic purposes have grasped a significant attention, of which the role of fecal microbiota transplantation (FMT) has been emphasized.
Methods
In the current study, we evaluated the effect of FMT on gut functions in Escherichia coli (E. coli) infection by using mice model. Moreover, we also investigated the subsequently dependent variables of infection, i.e., body weight, mortality, intestinal histopathology, and the expression changes in tight junction proteins (TJPs).
Results
The FMT effectively decreased weight loss and mortality to a certain extent with the restoration of intestinal villi that resulted in high histological scores for jejunum tissue damage (p < 0.05). The effect of FMT on alleviating the reduction of intestinal TJPs was also proved by immunohistochemistry analysis and mRNA expression levels. Moreover, the abundance of health-threatening bacteria, belonging to phylum Proteobacteria, family Enterobacteriaceae and Tannerellaceae, genus Escherichia-Shigella, Sphingomonas, Collinsella, etc., were significantly increased, whereas beneficial bacteria, belonging to phylum Firmicutes, family Lactobacillaceae, genus Lactobacillus were decreased in the gut of infected mice. Furthermore, we sought to investigate the association of clinical symptoms with FMT treatment with modulation in gut microbiota. According to beta diversity, the microbial community of gut microbiota results reflected the similarities between non-infected and FMT groups. The improvement of the intestinal microbiota in FMT group was characterized by the significant high level of beneficial microorganisms with the synergistic decrease of Escherichia-Shigella, Acinetobacter, and other taxa.
Conclusion
The findings suggest a beneficial host-microbiome correlation following fecal microbiota transplanatation for controlling gut infections and pathogens-associated diseases.
Funder
Guangxi Science and Technology Project Start-up fund of Nanjing Agricultural University Start-up Fund for Distinguished Scholars of Nanjing Agricultural Universitry
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
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