Author:
Mol Michael,Ardila Magda Stephania,Mol Bronwyn Ashleigh,Aliyu Habibu,Neumann Anke,de Maayer Pieter
Abstract
Abstract
Background
The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hydrogen gas (H2) through the water–gas shift (WGS) reaction. To date this process has been evaluated under controlled conditions, with gas feedstocks comprising carbon monoxide and variable proportions of air, nitrogen and hydrogen. Ultimately, an economically viable hydrogenogenic system would make use of industrial waste/synthesis gases that contain high levels of carbon monoxide, but which may also contain contaminants such as H2, oxygen (O2) and other impurities, which may be toxic to P. thermoglucosidasius.
Results
We evaluated the effects of synthesis gas (syngas) mimetic feedstocks on WGS reaction-driven H2 gas production by P. thermoglucosidasius DSM 6285 in small-scale fermentations. Improved H2 gas production yields and faster onset towards hydrogen production were observed when anaerobic synthetic syngas feedstocks were used, at the expense of biomass accumulation. Furthermore, as the WGS reaction is an anoxygenic process, we evaluated the influence of O2 perturbation on P. thermoglucosidasius hydrogenogenesis. O2 supplementation improved biomass accumulation, but reduced hydrogen yields in accordance with the level of oxygen supplied. However, H2 gas production was observed at low O2 levels. Supplementation also induced rapid acetate consumption, likely to sustain growth.
Conclusion
The utilisation of anaerobic syngas mimetic gas feedstocks to produce H2 and the relative flexibility of the P. thermoglucosidasius WGS reaction system following O2 perturbation further supports its applicability towards more robust and continuous hydrogenogenic operation.
Funder
South African CSIR-DSI Interprogramme Bursary scheme
Bundesministerium für Bildung und Forschung (BMBF) Bioökonomie International
Karlsruher Institut für Technologie (KIT)
Publisher
Springer Science and Business Media LLC
Reference45 articles.
1. IRENA. Global energy transformation: a roadmap to 2050. Abu Dhabi, 2018.
2. Elbeshbishy E, Dhar BR, Nakhla G, Lee HS. A critical review on inhibition of dark biohydrogen fermentation. Renew Sustain Energy Rev. 2017;79:656–68.
3. Abdalla AM, Hossain S, Nisfindy OB, Azad AT, Dawood M, Azad AK. Hydrogen production, storage, transportation and key challenges with applications: a review. Energy Convers Manage. 2018;165:602–27.
4. Hosseini SE, Wahid MA. Hydrogen production from renewable and sustainable energy resources: promising green energy carrier for clean development. Renew Sustain Energy Rev. 2016;57:850–66.
5. van Niel EW. Biological processes for hydrogen production. In: Hatti-Kaul R, Mamo G, Mattiasson B, editors. Anaerobes in biotechnology. Springer: Cham; 2016. p. 155–93.