Abstract
AbstractThe intention of this short primer is to raise your appetite for proper quantitative assessment of lung micro-structure. The method of choice for obtaining such data is stereology. Rooted in stochastic geometry, stereology provides simple and efficient tools to obtain quantitative three-dimensional information based on measurements on nearly two-dimensional microscopic sections. In this primer, the basic concepts of stereology and its application to the lung are introduced step by step along the workflow of a stereological study. The integration of stereology in your laboratory work will help to improve its quality. In a broader context, stereology may also be seen as a contribution to good scientific practice.
Funder
Deutsche Forschungsgemeinschaft
Else Kröner-Fresenius-Stiftung
Publisher
Springer Science and Business Media LLC
Reference49 articles.
1. Baddeley AJ, Gundersen HJG, Cruz-Orive LM. Estimation of surface area from vertical sections. J Microsc. 1986;142:259–76.
2. Baddeley A, Vedel Jensen EB. Stereology for statisticians. Boca Raton, FL: Chapman and Hall; 2005.
3. Bertram JF, Cruz-Orive LM, Evans SM, Hyde DM, Mayhew T, Ochs M, Tang Y, Nyengaard JR. Personal reflections on the life of Hans Jørgen Gottlieb Gundersen (1943–2021). Image Anal Stereol. 2021;40:49–61.
4. Boyce RW, Dorph-Petersen KA, Lyck L, Gundersen HJG. Design-based stereology: introduction to basic concepts and practical approaches for estimation of cell number. Toxicol Pathol. 2010;38:1011–25.
5. Braendgaard H, Gundersen HJG. The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Meth. 1986;18:39–78.
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