Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells

Author:

Trifunovic Sara,Smiljanić Katarina,Sickmann Albert,Solari Fiorella A.,Kolarevic Stoimir,Divac Rankov Aleksandra,Ljujic Mila

Abstract

Abstract Background Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. Results E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. Conclusions Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.

Funder

Mediterranean Institute for Life Sciences

Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja

Horizon 2020

Bundesministerium für Bildung und Forschung

Ministerium für Kultur und Wissenschaft des Landes Nordrhein-Westfalen

Regierender Bürgermeister von Berlin

Publisher

Springer Science and Business Media LLC

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