Author:
Galeotti Angela,Uomo Roberto,Spagnuolo Gianrico,Paduano Sergio,Cimino Roberta,Valletta Rosa,D’Antò Vincenzo
Abstract
Abstract
Background
Although the clinical use of miniscrews has been investigated on a large scale, little is known about their biocompatibility. Since low pH can affect corrosion resistance, the aim of this study was to evaluate the cytotoxic effect of orthodontic miniscrews in different pH conditions.
Methods
Four orthodontic miniscrews of stainless steel and grade IV and grade V titanium were immersed in a pH 7 and pH 4 saline solution for 1, 7, 14, 21, 28, and 84 days. Human osteogenic sarcoma cells (U2OS), permanent human keratinocytes (HaCat), and primary human gingival fibroblasts (HGF) were exposed to eluates, and the mitochondrial dehydrogenase activity was measured after 24 h to assess the cytoxicity. The results were analyzed using the Mann-Whitney U test (P < 0.05).
Results
When exposed to pH 7-conditioned eluates, the cell lines showed an even greater viability than untreated cells. On the contrary, the results revealed a statistically significant decrease in U2OS, HaCat, and HGF viability after exposure to eluates obtained at pH 4. Among the cell lines tested, HGF showed the most significant decrease of mitochondrial activity. Interestingly, grade V titanium miniscrews caused highest toxic effects when immersed at pH 4.
Conclusions
The results suggested that at pH 7, all the miniscrews are biocompatible while the eluates obtained at pH 4 showed significant cytotoxicity response. Moreover, different cell lines can produce different responses to miniscrew eluates.
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Kanomi R: Mini-implant for orthodontic anchorage. J Clin Orthod. 1997, 31: 763–67.
2. Prabhu J, Cousley RR: Current products and practice: bone anchorage devices in orthodontics. J Orthod. 2006, 33: 288–307.
3. Feldmann I, Bondemark L: Anchorage capacity of osseointegrated and conventional anchorage systems: a randomized controlled trial. Am J Orthod Dentofacial Orthop 2008, 133: 339. e19–28 e19–28
4. Eliades T, Bourauel C: Intraoral aging of orthodontic materials: the picture we miss and its clinical relevance. Am J Orthod Dentofacial Orthop. 2005, 127: 403–12.
5. D’Antò V, Uomo R, Paduano S, Laino A, Valletta R: Metal ion release from orthodontic appliances in vivo : a systematic review. Ortognatodonzia Italiana. 2007, 14: 235–41.
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