Author:
Zweers Jessica C,Barák Imrich,Becher Dörte,Driessen Arnold JM,Hecker Michael,Kontinen Vesa P,Saller Manfred J,Vavrová L'udmila,van Dijl Jan Maarten
Abstract
Abstract
Background
The Gram-positive bacterium Bacillus subtilis is an important producer of high quality industrial enzymes and a few eukaryotic proteins. Most of these proteins are secreted into the growth medium, but successful examples of cytoplasmic protein production are also known. Therefore, one may anticipate that the high protein production potential of B. subtilis can be exploited for protein complexes and membrane proteins to facilitate their functional and structural analysis. The high quality of proteins produced with B. subtilis results from the action of cellular quality control systems that efficiently remove misfolded or incompletely synthesized proteins. Paradoxically, cellular quality control systems also represent bottlenecks for the production of various heterologous proteins at significant concentrations.
Conclusion
While inactivation of quality control systems has the potential to improve protein production yields, this could be achieved at the expense of product quality. Mechanisms underlying degradation of secretory proteins are nowadays well understood and often controllable. It will therefore be a major challenge for future research to identify and modulate quality control systems of B. subtilis that limit the production of high quality protein complexes and membrane proteins, and to enhance those systems that facilitate assembly of these proteins.
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Reference186 articles.
1. Schallmey M, Singh A, Ward OP: Developments in the use of Bacillus species for industrial production. Can J Microbiol. 2004, 50: 1-17. 10.1139/w03-076.
2. Harwood CR: Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotechnol. 1992, 10: 247-256. 10.1016/0167-7799(92)90233-L.
3. Bron S, Meima R, van Dijl JM, Wipat A, Harwood CR: Molecular biology and genetics of Bacillus species. The Manual of industrial microbiology and biotechnology, 2nd edition . Edited by: Demain AL and Davies JE. 1999, USA, ASM press, 392-416.
4. Meima R, van Dijl JM, Holsappel S, Bron S: Expression systems in Bacillus. Expression technologies: current status and future trends . Edited by: Baneux F. 2004, Wymondham, UK, Horizon Scientific Press
5. Meima R, van Dijl JM: Protein secretion in Gram-positive bacteria. Protein Secretion Pathways in Bacteria. Edited by: Oudega B. 2003, Dordrecht, The Netherlands, Kluwer Academic Publisher, 271-296.
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