Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Abstract

AbstractGenetic resources available forArabidopsis thalianamake this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells fromArabidopsisleaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+and anion channels of intact stomatal guard cells to that achieved previously in work withViciaand tobacco guard cells.