Abstract
Abstract
Background
Channa punctata, Indian spotted snakehead, has a great economic value in south and south-east Asia being an important protein source for humans. Fish cultures are affected due to various bacterial and viral infections. Vibrio harveyi is a fish pathogenic bacteria which causes several outbreaks throughout the world and leads to huge mortalities. In this study, leaves of Eichhornia crassipes (water hyacinth) were used to investigate its immunostimulatory potential in Channa punctata.
Results
The immunostimulatory effects of water hyacinth leaves were studied in fish fed with 2.5% and 5% supplementary feed (experimental groups) in comparison to normal feed (control groups). Gas chromatography mass spectrometry (GC-MS) analysis of E. crassipes methanol extract showed presence of various components which have immunostimulatory, antioxidant, antibacterial, and anti-inflammatory activities. The antibacterial activity, antioxidant potential, and presence of phenol and flavonoids in methanol and ethanol extracts supported its use in fish feed. The healthy acclimatized fish were challenged with V. harveyi weekly. Liver function tests, alkaline phosphatase levels, and immunoglobulin content in the experimental groups were improved with respect to those in the positive control group. The spleen and head kidney were obtained at the final day of experiment, and macrophages were isolated; higher percentage of phagocytosis and phagocytic index indicated enhanced cell-mediated immune response in fish due to supplemented feed.
Conclusion
Plant-infused feed with leaves of E. crassipes can be recommended as a regular feed supplement to enhance fish immunity and disease resistance against the V. harveyi infection.
Publisher
Springer Science and Business Media LLC
Reference38 articles.
1. Ardo, L., Yin, G., Xu, P., Varadi, L., Szigeti, G., Jeney, Z., & Jeney, G. (2008). Chinese herbs (Astragalus membranaceus) and (Lonicera japonica) and boron enhances the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture, 275, 26–33.
2. Arvouet-Grand, A., Vennat, B., Pourrat, A., & Legret, P. (1994). Standardization of a propolis extract and identification of the main constituents. Journal de Pharmacie de Belgique, 49, 462–468.
3. Bartfay, W. J., Bartfay, E., & Johnson, J. G. (2012). Gram-negative and gram-positive antibacterial properties of whole plant extract of willow herb (Epilibium angustifolium). Biological Research for Nursing, 14, 85–89.
4. Bergier, I., Salis, S. M., Miranda, C. H. B., Ortega, E., & Luengo, C. A. (2012). Biofuel production from water hyacinth in the pantanal wetland. Ecohydrology & Hydrobiology, 12, 77–84.
5. Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25–30.
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献