Author:
Chen Weilan,Hu Xiaoling,Hu Li,Hou Xinyue,Xu Zhengyan,Yang Fanmin,Yuan Min,Chen Feifan,Wang Yunxiao,Tu Bin,Li Ting,Kang Liangzhu,Tang Shiwen,Ma Bingtian,Wang Yuping,Li Shigui,Qin Peng,Yuan Hua
Abstract
Abstract
Background:
Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown.
Findings:
We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-grain weight. Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation. MutMap-based gene cloning and a transgenic experiment demonstrated that WG3 encodes a GRAS protein. Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size. Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice. Collectively, our results reveal a new genetic and molecular mechanism for the regulation of grain size in rice by the WG3-DLT complex, and highlight the important functions of the GRAS protein complex in plants.
Conclusion:
WG3 functions directly in regulating grain size and BR signaling in rice.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Sichuan Province
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Soil Science,Agronomy and Crop Science
Cited by
5 articles.
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