Author:
Liao Yongxiang,Xiang Bing,Xue Zhenzhen,Ali Asif,Li Yong,Li Mengyuan,Wei Aiji,Xin Jialu,Guo Daiming,Liao Yingxiu,Tian Yunfeng,Zhao Zhixue,Xu Peizhou,Zhang Hongyu,Chen Xiaoqiong,Liu Yutong,Zhou Hao,Xia Duo,Du Kangxi,Wu Xianjun
Abstract
AbstractChlorophyll degradation is an important physiological process and is essential for plant growth and development. However, how chlorophyll degradation is controlled at the cellular and molecular level remains largely elusive. Pectin is a main component of the primary cell wall, and polygalacturonases (PGs) is a group of pectin-hydrolases that cleaves the pectin backbone and release oligogalacturonide. Whether and how PGs affect chlorophyll degradation metabolism and its association with ethylene (ETH) have not been reported before. Here, we report a novel function of PG in a mutant ‘high chlorophyll content1’ hcc1, which displayed a decrease in growth and yield. Our morphological, biochemical and genetic analyses of hcc1, knockout lines and complementation lines confirm the function of HCC1 in chlorophyll degradation. In hcc1, the PG activity, ETH content and D-galacturonic acid (D-GA) was significantly decreased and showed an increase in the thickness of the cell wall. Exogenous application of ETH and D-GA can increase ETH content and induce the expression of HCC1, which further can successfully induce the chlorophyll degradation in hcc1. Together, our data demonstrated a novel function of HCC1 in chlorophyll degradation via the ETH pathway.
Funder
Key Research and Development Program of Sichuan Province
Chengdu Science and Technology Bureau
Changde Science and Technology Bureau
Scientific research interest cultivation of Sichuan Agricultural University
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Soil Science,Agronomy and Crop Science
Reference56 articles.
1. Abe A, Kosugi S, Yoshida K, Natsume S, Takagi H, Kanzaki H, Matsumura H, Yoshida K, Mitsuoka C, Tamiru M (2012) Genome sequencing reveals agronomically important loci in rice using MutMap. Nat Biotechnol 30:174
2. Albersheim P, Darvill A, O’neill M, Schols H, Voragen AG. (1996) An hypothesis: the same six polysaccharides are components of the primary cell walls of all higher plants. Prog Biotechnol 14:47–55
3. Ali A, Wu T, Zhang H, Xu P, Zafar SA, Liao Y, Chen X, Zhou H, Liu Y, Wang W, Wu X (2022) A putative SUBTILISIN-LIKE SERINE PROTEASE 1 (SUBSrP1) regulates anther cuticle biosynthesis and panicle development in rice. J Adv Res 42:273–287
4. Anderson CT (2016) We be jammin’: an update on pectin biosynthesis, trafficking and dynamics. J Exp Bot 67:495–502
5. Atkinson RG, Schröder R, Hallett IC, Cohen D, MacRae EA (2002) Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion. Plant Physiol 129:122–133
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献