Author:
Chen Wei,Tang Liqun,Li Qianlong,Cai Yicong,Ahmad Shakeel,Wang Yakun,Tang Shengjia,Guo Naihui,Wei Xiangjin,Tang Shaoqing,Shao Gaoneng,Jiao Guiai,Xie Lihong,Hu Shikai,Sheng Zhonghua,Hu Peisong
Abstract
AbstractAs the source of isoprenoid precursors, the plastidial methylerythritol phosphate (MEP) pathway plays an essential role in plant development. Here, we report a novel rice (Oryza sativa L.) mutant ygl3 (yellow-green leaf3) that exhibits yellow-green leaves and lower photosynthetic efficiency compared to the wild type due to abnormal chloroplast ultrastructure and reduced chlorophyll content. Map-based cloning showed that YGL3, one of the major genes involved in the MEP pathway, encodes 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, which is localized in the thylakoid membrane. A single base substitution in ygl3 plants resulted in lower 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity and lower contents of isopentenyl diphosphate (IPP) compared to the wild type. The transcript levels of genes involved in the syntheses of chlorophyll and thylakoid membrane proteins were significantly reduced in the ygl3 mutant compared to the wild type. The phytochrome interacting factor-like gene OsPIL11 regulated chlorophyll synthesis during the de-etiolation process by directly binding to the promoter of YGL3 to activate its expression. The findings provides a theoretical basis for understanding the molecular mechanisms by which the MEP pathway regulate chloroplast development in rice.
Funder
Natural Science Foundation of Jiangxi Province
National Natural Science Foundation of China
National Science and Technology Major Project
National Key Research and Development Program of China
Science and Technology Program of Zhejiang Province
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Soil Science,Agronomy and Crop Science
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