Author:
Arnold Cosby G.,Konigsberg Iain,Adams Jason Y.,Sharma Sunita,Aggarwal Neil,Hopkinson Andrew,Vest Alexis,Campbell Monica,Boorgula Meher,Yang Ivana,Gignoux Christopher,Barnes Kathleen C.,Monte Andrew A.
Abstract
AbstractRT-PCR is the foremost clinical test for diagnosis of COVID-19. Unfortunately, PCR-based testing has limitations and may not result in a positive test early in the course of infection before symptoms develop. Enveloped RNA viruses, such as coronaviruses, alter peripheral blood methylation and DNA methylation signatures may characterize asymptomatic versus symptomatic infection. We used Illumina’s Infinium MethylationEPIC BeadChip array to profile peripheral blood samples from 164 patients who tested positive for SARS-CoV-2 by RT-PCR, of whom 8 had no symptoms. Epigenome-wide association analysis identified 10 methylation sites associated with infection and a quantile–quantile plot showed little inflation. These preliminary results suggest that differences in methylation patterns may distinguish asymptomatic from symptomatic infection.
Funder
National Heart, Lung, and Blood Institute
National Institutes of Health
Publisher
Springer Science and Business Media LLC
Subject
Drug Discovery,Genetics,Molecular Biology,Molecular Medicine
Reference8 articles.
1. Dong E, Du H, Gardner L. An interactive web-based dashboard to track covid-19 in real time. Lancet Infect Dis. 2020;20(5):533–4.
2. Wu Z, Mcgoogan JM. Characteristics of and important lessons from the coronavirus disease 2019 (Covid-19) outbreak in china: summary of a report of 72 314 cases from the chinese center for disease control and prevention. JAMA. 2020.
3. Kucirka LM, Lauer SA, Laeyendecker O, Boon D, Lessler J. Variation in false-negative rate of reverse transcriptase polymerase chain reaction-based SARS-CoV-2 tests by time since exposure. Ann Intern Med. 2020;173(4):262–7.
4. Menachery VD, Schäfer A, Burnum-Johnson KE, Mitchell HD, Eisfeld AJ, Walters KB, et al. MERS-CoV and H5N1 influenza virus antagonize antigen presentation by altering the epigenetic landscape. Proc Natl Acad Sci USA. 2018;115(5):E1012–21.
5. Konigsberg IR, Barnes B, Campbell M, Davidson E, Zhen Y, Pallisard O, et al. Host methylation predicts SARS-CoV-2 infection and clinical outcome. Commun Med. 2021;1(42):1–10.
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