Author:
Sharma Veena,Sharma Preeti
Abstract
Abstract
Background
Therapeutic potential of Prosopis cineraria has been extensively explored by many eminent researchers against various serious diseases but its activity against Lipopolysaccharide (endotoxin) is obscure. Therefore, present experimental investigation was conducted to unravel and analyze the anti-inflammatory potential of hydro-ethanol extract of Prosopis cineraria against LPS-induced inflammation in Swiss albino male mice.
Methods
Swiss Albino male mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with low and high dose (i.e. 100 mg/kg body weight and 300 mg/kg body weight respectively) of hydro-ethanol extract of stem-bark of Prosopis cineraria. The levels of cytokines (TNF-α, Prostaglandins E2, IL-6, NF-κBp65, IFN-γ and IL-10) were determined in liver homogenate. Nitric oxide generated due to LPS-induced toxicity was estimated by using Griess reagent.
Results
The results demonstrated that the plant extract suppressed the over-expression and altered levels of cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- γ, Prostaglandin E2 and IL-10.
Conclusion
The present research work unravelled the alleviating potential of Prosopis cineraria against LPS-induced inflammation by modulating the expression of cytokines.
Publisher
Springer Science and Business Media LLC
Subject
General Earth and Planetary Sciences,General Environmental Science
Reference33 articles.
1. Callery MP, Kamei T, Flye MW. Kupffer cell blockade increases mortality during intra-abdominal sepsis despite improving systemic immunity. Arch Surg. 1990;125(1):36–41.
2. Rietschel ET, Holst O, Brade L, Muller-Loennies S, Mamat U, Zahringer U, et al. Bacterial endotoxin: chemical constitution, biological recognition, host response, and immunological detoxification. Curr Top Microbiol Immunol. 1996;216:39–81.
3. Berczi I, Bertok I, Bereznai T. Comparative studies on the toxicity of Escherichia coli lipopolysaccharide endotoxin in various animal species. Can J Microbiol. 1966;12(5):1070–1.
4. Park HS, Jung HY, Park EY, Kim J, Lee WJ, Bae YS. Cutting edge: direct interaction of TLR4 with NAD(P) H oxidase 4 isozyme is essential for lipopolysaccharide-induced production of reactive oxygen species and activation of NF-kappa B. J Immunol. 2004;173(6):3589–93.
5. Weiss SJ. Tissue destruction by neutrophils. N Engl J Med. 1989;320(6):365–76.
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