Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus

Abstract

AbstractBackgroundIn fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein degradation. F-box proteins are well studied in different model plants however, their functions in the fungi are not clear yet. This study aimed to identify the genes involved in protein degradation for disease development in theMacrophomina phaseolinafungus.ResultsIn this research,in silicostudies were done to understand the distribution of F-box proteins in pathogenic fungi includingMacrophomina phaseolinafungus. Genome-wide analysis indicates thatM. phaseolinafungus contained thirty-one F-box proteins throughout its chromosomes. In addition, there are 17, 37, 16, and 21 F-box proteins have been identified fromPuccinia graminis, Colletotrichum graminicola, Ustilago maydis, andPhytophthora infestans, respectively. Analyses revealed that selective fungal genomes contain several additional functional domains along with F-box domain. Sequence alignment showed the substitution of amino acid in several F-box proteins; however, gene duplication was not found among these proteins. Phylogenetic analysis revealed that F-box proteins having similar functional domain was highly diverse form each other showing the possibility of various function. Analysis also found that MPH_00568 and MPH_05531 were closely related to rice blast fungus F-box protein MGG_00768 and MGG_13065, respectively, may play an important role for blast disease development.ConclusionThis genome-wide analysis of F-box proteins will be useful for characterization of candidate F-box proteins to understand the molecular mechanisms leading to disease development ofM. phaseolinain the host plants.