Author:
Chen Ching-Wen,Liu Chin-San,Chiu Ing-Ming,Shen Shih-Cheng,Pan Hung-Chuan,Lee Kun-Hsiung,Lin Shinn-Zong,Su Hong-Lin
Abstract
Abstract
Background
Neural induction is a complex process and the detailed mechanism of FGF-induced neurogenesis remains unclear.
Methods
By using a serum-free neural induction method, we showed that FGF1 dose-dependently promoted the induction of Sox1/N-cadherin/nestin triple positive cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells.
Results
We demonstrated that FGF1, FGF2, and FGF4, but not FGF8b, enhanced this neurogenesis. Especially, FGF-enhanced neurogenesis is not mediated through the rescue of the apoptosis or the enhancement of the proliferation of Sox1+ cells. We further indicated that the inactivation of c-Jun N-terminal kinase-1 (JNK-1) and extracellular signal-related kinase-2 (ERK-2), but not p38 mitogen-activated protein kinase (MAPK), inhibited the neural formation through the inhibition of ES differentiation, but not through the formation of endomesodermal cells.
Conclusions
These lines of evidence delineated the roles of FGF downstream signals in the early neural differentiation of ES cells.
Publisher
Springer Science and Business Media LLC
Subject
Pharmacology (medical),Biochemistry (medical),Cell Biology,Clinical Biochemistry,Molecular Biology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
27 articles.
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