Author:
He Lingxiao,Liu Huabing,Cheng Changhe,Xu Min,He Lei,Li Lihua,Yao Jian,Zhang Wenjun,Zhai Zhengguang,Luo Qinzhan,Sun Jutao,Yang Tiezhao,Xu Shixiao
Abstract
Abstract
Background
Amber-like compounds form in tobacco (Nicotiana tabacum) during leaf curing and impact aromatic quality. In particular, cis-abienol, a polycyclic labdane-related diterpenoid, is of research interest as a precursor of these compounds. Glandular trichome cells specifically express copalyl diphosphate synthase (NtCPS2) at high levels in tobacco, which, together with NtABS, are major regulators of cis-abienol biosynthesis in tobacco.
Results
To identify the genes involved in the biosynthesis of cis-abienol in tobacco, we constructed transgenic tobacco lines based on an NtCPS2 gene-knockdown model using CRISPR/Cas9 genome-editing technology to inhibit NtCPS2 function in vitro. In mutant plants, cis-abienol and labdene diol contents decreased, whereas the gibberellin and abscisic acid (ABA) contents increased compared with those in wild-type tobacco plants. RNA sequencing analysis revealed the presence of 9514 differentially expressed genes (DEGs; 4279 upregulated, 5235 downregulated) when the leaves of wild-type and NtCPS2-knockdown tobacco plants were screened. Among these DEGs, the genes encoding cis-abienol synthase, ent-kaurene oxidase, auxin/ABA-related proteins, and transcription factors were found to be involved in various biological and physiochemical processes, including diterpenoid biosynthesis, plant hormone signal transduction, and plant-pathogen interactions.
Conclusions
The present study provides insight into the unique transcriptome profile of NtCPS2 knockdown tobacco, allowing for a better understanding of the biosynthesis of cis-abienol in tobacco.
Funder
China National Tobacco Corporation Henan company
Henan Tobacco Corporation XuChang Company
Guizhou Tobacco Corporation Guiyang company
Hunan Tobacco Corporation Changsha Company
Guangxi Zhuang Autonomous Region Tobacco Corporation Baise Company
Publisher
Springer Science and Business Media LLC
Cited by
6 articles.
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