Author:
Tian Chang-Xu,Lin Xing-Hua,Zhou Da-Yan,Chen Yu,Shen Yi-Jun,Ye Ming-Hui,Duan Cun-Yu,Zhang Yu-Lei,Yang Bin-Lan,Deng Si-Ping,Zhu Chun-Hua,Li Guang-Li
Abstract
Abstract
Background
Hong Kong catfish (Clarias fuscus) is an ecologically and economically important species that is widely distributed in freshwater regions of southern China. Hong Kong catfish has significant sexual growth dimorphism. The genome assembly of the Hong Kong catfish would facilitate study of the sex determination and evolution mechanism of the species.
Results
The first high-quality chromosome-level genome of the Hong Kong catfish was constructed. The total genome was 933.4 Mb, with 416 contigs and a contig N50 length of 8.52 Mb. Using high-throughput chromosome conformation capture (Hi-C) data, the genome assembly was divided into 28 chromosomes with a scaffold N50 length of 36.68 Mb. A total of 23,345 protein-coding genes were predicted in the genome, and 94.28% of the genes were functionally annotated in public databases. Phylogenetic analysis indicated that C. fuscus and Clarias magur diverged approximately 63.7 million years ago. The comparative genome results showed that a total of 60 unique, 353 expanded and 851 contracted gene families were identified in Hong Kong catfish. A sex-linked quantitative trait locus identified in a previous study was located in a sex-determining region of 30.26 Mb (0.02 to 30.28 Mb) on chromosome 13 (Chr13), the predicted Y chromosome. This QTL region contained 785 genes, of which 18 were identified as sex-related genes.
Conclusions
This study is the first to report the chromosome-level genome assembly of Hong Kong catfish. The study provides an excellent genetic resource that will facilitate future studies of sex determination mechanisms and evolution in fish.
Funder
Guangdong Basic and Applied Basic Research Foundation
Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province
National College Students Innovation and Entrepreneurship Training Program
Special Funds for the Cultivation of Guangdong College Students’ Scientific and Technological Innovation
Self-financing Project of Guangxi Agricultural Science and Technology
Publisher
Springer Science and Business Media LLC
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献