Abstract
Abstract
Background
Genomic comparison of Mycoplasma synoviae vaccine strain MS-H and the MS-H parental strain 86,079/7NS established a preliminary profile of genes related to attenuation of MS-H. In this study we aimed to identify the stability of mutations found in MS-H after passage in experimental or field chickens, and to evaluate if any reverse mutation may be associated with changes in characteristics of MS-H in vitro or in vivo.
Results
Whole genome sequence analysis of 5 selected MS-H field reisolates revealed that out of 32 mutations reported previously in MS-H, 28 remained stable, while four found to be reversible to the wild-type. Each isolate possessed mutations in one to three of the genes obg, oppF1 and gap and/or a non-coding region. Examination of the 4 reversible mutations by protein modeling predicted that only two of them (in obg and oppF1 genes) could potentially restore the function of the respective protein to that of the wild-type.
Conclusions
These results suggest that the majority of the MS-H mutations are stable after passage in vaccinated chickens. Characterisation of stable mutations found in MS-H could be utilised to develop rapid diagnostic techniques for differentiation of vaccine from field strains or ts- MS-H reisolates.
Publisher
Springer Science and Business Media LLC
Reference39 articles.
1. Catania S, Bilato D, Gobbo F, Granato A, Terregino C, Iob L, et al. Treatment of eggshell abnormalities and reduced egg production caused by Mycoplasma synoviae infection. Avian Dis. 2010;54(2):961–4.
2. Ferguson-Noel N, Noormohammadi AH. Mycoplasma synoviae infection. In: Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair VL, editors. Diseases of Poultry. 13th ed. Hoboken: Wiley-Blackwell; 2013. p. 875.
3. Morrow CJ, Markham JF, Whithear KG. Production of temperature-sensitive clones of Mycoplasma synoviae for evaluation as live vaccines. Avian Dis. 1998;42(4):667–70.
4. Cruz-Vera LR, Toledo I, Hernandez-Sanchez J, Guarneros G. Molecular basis for the temperature sensitivity of Escherichia coli pth (Ts). J Bacteriol. 2000;182(6):1523–8.
5. Haga T, Murayama N, Shimizu Y, Saito A, Sakamoto T, Morita T, et al. Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model. Comp Immunol Microb. 2009;32(5):395–406.
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