Genome-wide association study for stalk lodging resistance related traits in maize (Zea mays L.)-Reference-Cited by-同舟云学术

Genome-wide association study for stalk lodging resistance related traits in maize (Zea mays L.)

Published:2024-01-02 Issue:1 Volume:25 Page:
ISSN:1471-2164
Container-title:BMC Genomics
language:en
Short-container-title:BMC Genomics

Author:

Wang Bangtai,Yang Meili,Guo Hua,Wang Jing,Wang Zhihong,Lu Hongwei,Qin Guiwen,Chen Jiafa

Abstract

Abstract Background The stalk traits stalk diameter, stalk length, rind penetrometer resistance and dry matter content are important indicators for measuring lodging resistance. Results In this study, 377 inbred lines were used as the basic materials, and four stalk-related traits including stalk diameter, stalk length, rind penetrometer resistance and dry matter content of the third segment of maize, were investigated at the tasseling, grain filling, and maturity stages. 461,053 high-quality SNPs which were obtained by whole genome resequencing were used for genome-wide association study. As a result of mixed linear model analysis (P < 9.77 × 10–6), 29 significant SNPs related to traits were detected, accounting for 7.19% -15.03% of phenotypic variation, among which 4, 1, 4 and 20 SNPs were found related to rind penetrometer resistance, stalk diameter, stalk length, and dry matter content respectively. Most candidate genes are related to plant element structure, signal transduction mechanisms, inorganic ion transport and metabolism, nucleotide transport and metabolism, and transporter enzyme families. Comparing mixed linear model with generalized linear model, a total of 12 candidate genes were detected repeatedly, during which the candidate gene Zm00001d014449 were detected 5 times, with a phenotypic variation interpretation rate of 9.95% -10.84%. This gene is mainly expressed in cells with active cell division and tissue differentiation, and is involved in the formation of stalk vascular bundles and the synthesis of cell walls. Another candidate gene, Zm00001d005300, encodes the transcription factor MYB44, which regulates the dependence of salt stress signal phosphorylation, can effectively inhibit the accumulation of destructive reactive oxygen species, and has a certain resistance to non-biotic stress. In addition, this study also found that 10 unknown functional genes can be further Functional verification. Conclusions This study helps to deepen the understanding of the genetic basis of traits related to maize stalk lodging resistance, and provides theoretical guidance for future maize lodging resistance breeding.

Funder

Henan Provincial Key Science and Technology Project "Creation of Germplasm Resources and New Variety Breeding and Application of High-Yield and Multi-Resistance Maize"

Henan Provincial Science and Technology Project "Analysis of Genetic Basis and Application of Stalk Quality Traits by GWAs"

Publisher

Springer Science and Business Media LLC

Link

https://link.springer.com/content/pdf/10.1186/s12864-023-09917-x.pdf

Reference29 articles.

1. Ma YJ, Bao JX, Gao YX, Li YN, Qin WX, Wang YB, Long Y, Li JP, Dong ZY, Wan XY. Genome- wide association analysis of plant height and ear height related traits in maize. Acta Agronomica Sinica. 2023;49(03):647–61 (In Chinese).

2. Ma QM, Pei YB, Chen DB, Song XY. Genome-wide association analysis of maize stalk anti-thrust. Scientia Agricultura Sinical. 2017;50(24):4671–8 (In Chinese).

3. Ren AR, Wu LF, Guan HH, Yang S, Jian LQ, Liu YF, Du YQ, Wang J, Guo JJ, Chen JT. Stalk fiber related traits and lodging resistance correlation analysis under different planting density in maize. J Plant Genet Resour. 2017;18(4):653–64 (In Chinese).

4. Xie LY. Genome-wide association studies and important gene mining of maize stalk strength related traits, Ph. D. Dissertation of Shandong Agricultural University, 2021 (In Chinese)

5. Liu XG. Genomic selection for yield-relevant traits and stalk strength in maize, Postdoctoral Thesis of the Chinese Academy of Agricultural Sciences, 2020. (In Chinese)