Author:
Kumar Deepesh,Ramkumar M. K.,Dutta Bipratip,Kumar Ajay,Pandey Rakesh,Jain Pradeep Kumar,Gaikwad Kishor,Mishra Dwijesh C.,Chaturvedi K. K.,Rai Anil,Solanke Amolkumar U.,Sevanthi Amitha Mithra
Abstract
AbstractTo combat drought stress in rice, a major threat to global food security, three major quantitative trait loci for ‘yield under drought stress’ (qDTYs) were successfully exploited in the last decade. However, their molecular basis still remains unknown. To understand the role of secondary regulation by miRNA in drought stress response and their relation, if any, with the three qDTYs, the miRNA dynamics under drought stress was studied at booting stage in two drought tolerant (Sahbaghi Dhan and Vandana) and one drought sensitive (IR 20) cultivars. In total, 53 known and 40 novel differentially expressed (DE) miRNAs were identified. The primary drought responsive miRNAs were Osa-MIR2919, Osa-MIR3979, Osa-MIR159f, Osa-MIR156k, Osa-MIR528, Osa-MIR530, Osa-MIR2091, Osa-MIR531a, Osa-MIR531b as well as three novel ones. Sixty-one target genes that corresponded to 11 known and 4 novel DE miRNAs were found to be co-localized with the three qDTYs, out of the 1746 target genes identified. We could validate miRNA-mRNA expression under drought for nine known and three novel miRNAs in eight different rice genotypes showing varying degree of tolerance. From our study, Osa-MIR2919, Osa-MIR3979, Osa-MIR528, Osa-MIR2091-5p and Chr01_11911S14Astr and their target genes LOC_Os01g72000, LOC_Os01g66890, LOC_Os01g57990, LOC_Os01g56780, LOC_Os01g72834, LOC_Os01g61880 and LOC_Os01g72780 were identified as the most promising candidates for drought tolerance at booting stage. Of these, Osa-MIR2919 with 19 target genes in the qDTYs is being reported for the first time. It acts as a negative regulator of drought stress tolerance by modulating the cytokinin and brassinosteroid signalling pathway.
Publisher
Springer Science and Business Media LLC
Reference99 articles.
1. Childs, N. RCS-20H, U.S. Department of Agriculture, Economic Research Service, August 16, 2022
2. Bartels D, Sunkar R. Drought and salt tolerance in plants. Crit Rev Plant Sci. 2005;24(1):23–58.
3. Berahim Z, Dorairaj D, Omar MH, Saud HM, Ismail MR. Spermine mediated improvements on stomatal features, growth, grain filling and yield of rice under differing water availability. Sci Rep. 2021;11(1):10669.
4. Liu C, Chen K, Zhao X, Wang X, Shen C, Zhu Y, Dai M, Qiu X, Yang R, Xing D, Pang Y. Identification of genes for salt tolerance and yield-related traits in rice plants grown hydroponically and under saline field conditions by genome-wide association study. Rice. 2019;12:1–3.
5. Abobatta WF. Drought adaptive mechanisms of plants—A review. Advances in Agriculture and Environmental Science. 2019;2(1):62–5.