Author:
Tang Yanyan,Li Xiaoting,Hu Changli,Qiu Xiaochen,Li Jingjing,Li Xin,Zhu Hong,Wang Jingshan,Sui Jiongming,Qiao Lixian
Abstract
Abstract
Background
The cultivated peanut (Arachis hypogaea L., AABB) is an allotetraploid hybrid between two diploid peanuts, A. duranensis (AA genome) and A. ipaensis (BB genome). Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons with high copy numbers, play important roles in genome evolution and diversification. AhMITE1, a member of the MITE family of transposons, but information on the peanut genomes is still limited. Here, we analyzed AhMITE1, AuMITE1 and ApMITE1 in the cultivated (A. hypogaea) and two wild peanut (A. duranensis and A. ipaensis) genomes.
Results
The cultivated and the two wild peanut genomes harbored 142, 14 and 21 AhMITE1, AuMITE1 and ApMITE1 family members, respectively. These three family members exhibited highly conserved TIR sequences, and insertions preferentially occurred within 2 kb upstream and downstream of gene-coding and AT-rich regions. Phylogenetic and pairwise nucleotide diversity analysis showed that AhMITE1 and ApMITE1 family members have undergone one round of amplification bursts during the evolution of the peanut genome. PCR analyses were performed in 23 peanut varieties and demonstrated that AhMITE1 is an active transposon and that hybridization or chemical mutagenesis can promote the mobilization of AhMITE1.
Conclusions
AhMITE1, AuMITE1 and ApMITE1 family members were identified based on local BLAST search with MAK between the cultivated and the two wild peanut genomes. The phylogenetic, nucleotide diversity and variation copy numbers of AhMITE1, AuMITE1 and ApMITE1 members provides opportunities for investigating their roles during peanut evolution. These findings will contribute to knowledge on diversity of AhMITE1, provide information about the potential impact on the gene expression and promote the development of DNA markers in peanut.
Publisher
Springer Science and Business Media LLC
Reference55 articles.
1. Food and Agriculture Organization (FAO, 2020), Food and Agricultural Organization of the United Nations, FAO statistical database. In http://www.fao.org/.
2. Hammons RO. The Groundnut Crop: A scientific basis for improvement. In: Smartt J, editor. The origin and history of the groundnut. London: Chapman and Hall; 1994. p. 24–42.
3. Koilkonda P, Sato S, Tabata S, Shirasawa K, Hirakawa H, et al. Large-scale development of expressed sequence tag-derived simple sequence repeat markers and diversity analysis in Arachis spp. Mol Breeding. 2011;30(1):125–38.
4. Bertioli DJ, Jenkins J, Clevenger J, Dudchenko O, Gao D, Seijo G, et al. The genome sequence of segmental allotetraploid peanut Arachis hypogaea. Nat Genet. 2019;51(5):877–84.
5. Zhuang W, Chen H, Yang M, Wang J, Pandey MK, Zhang C, et al. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication. Nat Genet. 2019;51(5):865–76.
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