Author:
Cao Xianmei,Zhao Ruibai,Wang Hongxing,Zhang Huaiwen,Zhao Xue,Khan Latif Ullah,Huang Xi
Abstract
Abstract
Background
Areca palm (Areca catechu L.) is an important commercial crop in southeast Asia, but its cultivation is threatened by yellowing leaf disease (YLD). Areca palm velarivirus 1 (APV1) was recently associated with YLD, but little is known regarding its population and genetic diversity. To assess the diversity of YLD, the APV1 genome was sequenced in YLD samples collected from different sites in Hainan.
Results
Twenty new and complete APV1 genomes were identified. The APV1 isolates had highly conserved sequences in seven open reading frames (ORFs; > 95% nucleotide [nt] identity) at the 3′ terminal, but there was diversity (81–87% nt identity) in three ORFs at the 5′ terminal. Phylogenetic analysis divided the APV1 isolates into three phylogroups, with 16 isolates (> 70%) in phylogroup A. Mixed infections with different genotypes in the same tree were identified; this was closely correlated with higher levels of genetic recombination.
Conclusions
Phylogroup A is the most prevalent APV1 genotype in areca palm plantations in Hainan, China. Mixed infection with different genotypes can lead to genomic recombination of APV1. Our data provide a foundation for accurate diagnostics, characterization of etiology, and elucidation of the evolutionary relationships of APV1 populations.
Publisher
Springer Science and Business Media LLC
Reference31 articles.
1. Manimekalai R, Deeshma KP, Manju KP, Soumya VP, Sunaiba M, Nair S, et al. Molecular marker-based genetic variability among Yellow Leaf Disease (YLD) resistant and susceptible arecanut (Areca catechu L.) genotypes. Indian J Hortic. 2012;69(4):455–61.
2. Nayar R, Seliskar CE. Mycoplasma like organisms associated with yellow leaf disease of Areca catechu L. Eur J For Pathol. 1978;8(2):125–8.
3. Ramaswamy M, Nair S, Soumya VP, Thomas GV. Phylogenetic analysis identifies a ‘Candidatus Phytoplasma oryzae’-related strain associated with yellow leaf disease of areca palm (Areca catechu L.) in India. Int J Syst Evol Microbiol. 2013;63(Pt 4):1376–82.
4. Luo DQ, Chen MR, Ye SB, Tsai JH. Identification of pathogens of yellow leaf disease of arecanut in Hainan Island. Chin J Trop Crops. 2001;22(2):43–6.
5. Che HY, Wu CT, Fu RY, Wen YS, Ye SB, Luo DQ. Molecular identification of pathogens from arecanut yellow leaf disease in Hainan. Chin J Trop Crops. 2010;31(1):83–7.
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