Abstract
Abstract
Background
Olfactory systems take on important tasks to distinguish salient information from a complex olfactory environment, such as locating hosts, mating, aggression, selecting oviposition sites, and avoiding predators. The olfactory system of an adult insect consists of two pairs of main olfactory appendages on the head, the antennae, and the palps, which are covered with sensilla. Benzothiazole and 1-octen-3-ol could elicit oviposition behavior in gravid B. dorsalis are regarded as oviposition stimulants. However, the mechanism for how B. dorsalis percepts benzothiazole and 1-octen-3-ol still remains unknown.
Results
We conducted a comparative analysis of the antennal transcriptomes in different genders of B. dorsalis using Illumina RNA sequencing (RNA-seq). We identified a total of 1571 differentially expressed genes (DEGs) among the two sexes, including 450 female-biased genes and 1121 male-biased genes. Among these DEGs, we screened out 24 olfaction-related genes and validated them by qRT-PCR. The expression patterns of these genes in different body parts were further determined. In addition, we detected the expression profiles of the screened female-biased chemosensory genes in virgin and mated female flies. Furthermore, the oviposition stimulants-induced expression profilings were used to identify chemosensory genes potentially responsible for benzothiazole and 1-octen-3-ol perception in this fly.
Conclusions
The results from this study provided fundamental data of chemosensory DEGs in the B. dorsalis antenna. The odorant exposure assays we employed lay a solid foundation for the further research regarding the molecular mechanism of benzothiazole and 1-octen-3-ol mediated oviposition behavior in B. dorsalis.
Funder
National Natural Science Foundation of China
Earmarked Fund for Modern Agro-industry Technology Research System
Publisher
Springer Science and Business Media LLC
Reference43 articles.
1. Zhao S, Xing Z, Liu Z, Liu Y, Liu X, Chen Z, Li J, Yan R. Efficient somatic and germline genome engineering of Bactrocera dorsalis by the CRISPR/Cas9 system. Pest Manag Sci. 2019;75(7):1921–32.
2. Ohno S, Tamura Y, Haraguchi D, Matsuyama T, Kohama T. Re-invasions by Bactrocera dorsalis complex (Diptera: Tepritidae) occurred after its eradication in Okinawa, Japan, and local differences found in the frequency and temporal patterns of invasions. Appl Entomol Zool. 2009;44(4):643–54.
3. Lu XP, Wang LL, Huang Y, Dou W, Chen CT, Wei D, Wang JJ. The epsilon glutathione S-transferases contribute to the malathion resistance in the oriental fruit fly, Bactrocera dorsalis (Hendel). Comp Biochem Physiol. 2016;180:40–8.
4. Jin T, Zeng L, Lin YY, Lu YY, Liang GW. Insecticide resistance of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in mainland China. Pest Manag Sci. 2011;67(3):370–6.
5. Haney S, Saha D, Raman B, Bazhenov M. Differential effects of adaptation on odor discrimination. J Neurophysiol. 2018;120(1):171–85.
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献