Microfluidics-based strategies for molecular diagnostics of infectious diseases

Author:

Wang Xin,Hong Xian-Zhe,Li Yi-Wei,Li Ying,Wang Jie,Chen Peng,Liu Bi-Feng

Abstract

AbstractTraditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Natural Science Foundation of Hubei Province

the Fundamental Research Funds for Central Universities, HUST

Publisher

Springer Science and Business Media LLC

Subject

General Medicine

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