Author:
Yang Cheng,Zhang Yan,Yan Mingze,Wang Jiahao,Wang Jiaming,Wang Muhong,Xuan Yuhong,Cheng Haiyue,Ma Jiaao,Chai Cuicui,Li Mingzhe,Yu Zhiwei
Abstract
Abstract
Background
Cancer associated fibroblasts (CAFs) can remodel tumor microenvironment by secreting exosomes. This study aimed to investigate the role of exosomes derived from cancer-associated fibroblasts in colorectal cancer (CRC) progression.
Methods
Circular RNA (circRNA) array was used to identify differentially expressed circRNAs in exosomes from normal fibroblasts (NFs) and CAFs, and confirmed one differentially expressed circRNA circ_0067557 by real-time PCR. The effect of circ_0067557 on proliferation, metastasis, chemoresistance and apoptosis was verified by wound heal, tranwell, CCK8, sphere-forming and flow cytometry assay.
Results
Circ_0067557 expression in exosomes from CAFs was higher than those from NFs. CAF-derived exosomes promoted the proliferation, migration, invasion and chemoresistance of CRC cells while suppressed apoptosis. Silencing of circ_0067557 inhibited malignant phenotypes of CRC cells by targeting Lin28A and Lin28B. Moreover, CAF-derived exosomes enhanced the growth of CRC xenograft tumors.
Conclusion
Circ_0067557/Lin28A and Lin28B signal axis may be a potential therapy target for CRC.
Publisher
Springer Science and Business Media LLC
Reference38 articles.
1. Dekker E, Tanis PJ, Vleugels JLA, Kasi PM, Wallace MB. Colorectal cancer. Lancet. 2019;394(10207):1467–80.
2. Zhang W, Sun R, Zhang Y, Hu R, Li Q, Wu W, et al. Cabazitaxel suppresses colorectal cancer cell growth via enhancing the p53 antitumor pathway. FEBS Open Bio. 2021;11(11):3032–50.
3. Lu J, Traub B, Kornmann M. The role of interleukin 13 receptor alpha 2 in inflammatory bowel disease and colorectal cancer. Translational Surg Oncol. 2023;1(1):3–9.
4. Ishaque N, Abba ML, Hauser C, Patil N, Paramasivam N, Huebschmann D, et al. Whole genome sequencing puts forward hypotheses on metastasis evolution and therapy in colorectal cancer. Nat Commun. 2018;9(1):4782.
5. Picard E, Verschoor CP, Ma GW, Pawelec G. Relationships between Immune landscapes, genetic subtypes and responses to Immunotherapy in Colorectal Cancer. Front Immunol. 2020;11:369.
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