Author:
Cortleven Anne,Noben Jean-Paul,Valcke Roland
Abstract
Abstract
Background
Cytokinin is a plant hormone that plays a crucial role in several processes of plant growth and development. In recent years, major breakthroughs have been achieved in the elucidation of the metabolism, the signal perception and transduction, as well as the biological functions of cytokinin. An important activity of cytokinin is the involvement in chloroplast development and function. Although this biological function has already been known for 50 years, the exact mechanisms remain elusive.
Results
To elucidate the effects of altered endogenous cytokinin content on the structure and function of the chloroplasts, chloroplast subfractions (stroma and thylakoids) from transgenic Pssu-ipt and 35S:CKX1 tobacco (Nicotiana tabacum) plants with, respectively, elevated and reduced endogenous cytokinin content were analysed using two different 2-DE approaches. Firstly, thykaloids were analysed by blue-native polyacrylamide gel electrophoresis followed by SDS-PAGE (BN/SDS-PAGE). Image analysis of the gel spot pattern thus obtained from thylakoids showed no substantial differences between wild-type and transgenic tobacco plants. Secondly, a quantitative DIGE analysis of CHAPS soluble proteins derived from chloroplast subfractions indicated significant gel spot abundance differences in the stroma fraction. Upon identification by MALDI-TOF/TOF mass spectrometry, these proteins could be assigned to the Calvin-Benson cycle and photoprotective mechanisms.
Conclusion
Taken together, presented proteomic data reveal that the constitutively altered cytokinin status of transgenic plants does not result in any qualitative changes in either stroma proteins or protein complexes of thylakoid membranes of fully developed chloroplasts, while few but significant quantitative differences are observed in stroma proteins.
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Biochemistry
Reference55 articles.
1. Mok MC: Cytokinins and plant development. Edited by: Mok DWS, Mok MC. Cytokinins: Chemistry, Activity and Function, CRC Press; 1994:57–79.
2. Werner T, Schmülling T: Cytokinin action in plant development. Curr Opin Plant Biol 2009, 12: 527–538. 10.1016/j.pbi.2009.07.002
3. Sakakibara H: Cytokinins: activity, biosynthesis and translocation. Annu Rev Biol 2006, 57: 431–449. 10.1146/annurev.arplant.57.032905.105231
4. Müller B, Sheen J: Arabidopsis cytokinin signalling pathway. STKE 2007. 407/cm5
5. Argueso CT, Ferreira FJ, Kieber JJ: Environmental perception avenues: the interaction of cytokinin and environmental response pathways. Plant Cell Environ 2009, 32: 1147–1160. 10.1111/j.1365-3040.2009.01940.x
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