Author:
D'Andrea Gabriele,Lizzi Anna R,Venditti Sara,Di Francesco Laura,Giorgi Alessandra,Mignogna Giuseppina,Oratore Arduino,Bozzi Argante
Abstract
Abstract
In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Biochemistry
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