Author:
Abdullah-Soheimi Siti S,Lim Boon-Kiong,Hashim Onn H,Shuib Adawiyah S
Abstract
Abstract
Background
Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of new noninvasive protocol for early diagnosis and screening purposes. In the present study, urine samples from patients with ovarian carcinoma were analysed by two-dimensional gel electrophoresis and the profiles generated were compared to those similarly obtained from age-matched cancer negative women.
Results
Significant reduced levels of CD59, kininogen-1 and a 39 kDa fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), and enhanced excretion of a 19 kDa fragment of albumin, were detected in the urine of patients with ovarian carcinoma compared to the control subjects. The different altered levels of the proteins were confirmed by Western blotting using antisera and a lectin that bind to the respective proteins.
Conclusion
CD59, kininogen-1 and fragments of ITIH4 and albumin may be used as complementary biomarkers in the development of new noninvasive protocols for diagnosis and screening of ovarian carcinoma.
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Biochemistry
Reference32 articles.
1. Lim GCC, Yahya H: Second Report of the National Cancer Registry, Cancer Incidence in Malaysia. National Cancer Registry, Ministry of Health, Kuala Lumpur; 2003.
2. Sasaroli D, Coukos G, Scholler N: Beyond CA125: the coming of age of ovarian cancer biomarkers. Are we there yet? Biomark Med 2009, 3: 275–288. 10.2217/bmm.09.21
3. Havrilesky LJ, Sanders GD, Kulasingam S, Myers ER: Reducing ovarian cancer mortality through screening: is it possible, and can we afford it? Gynecol Oncol 2008, 111: 179–187. 10.1016/j.ygyno.2008.07.006
4. Rapkiewicz AV, Espina V, Petricoin EF, Liotta LA: Biomarkers of ovarian tumours. Eur J Cancer 2004, 40: 2604–2612. 10.1016/j.ejca.2004.05.021
5. Pang WW, Abdul-Rahman PS, Wan-Ibrahim WI, Hashim OH: Can the acute-phase reactant proteins be used as cancer biomarkers? Int J Biol Markers 2010, 25: 1–11.