Innovative approach for semi‐continuous production of puerarin palmitate via transesterification with novel immobilized lipase

Abstract

Abstract Background Puerarin, a flavonoid abundantly found in the roots of Pueraria lobata, exhibits antioxidant, anti-cancer, anti-inflammatory, and anti-aging properties. Despite its numerous benefits, the low bioavailability of puerarin hinders its widespread industrial application. To improve this, flavonoid acylation is required. Lipase is a biocatalyst primarily used for flavonoid acylation; immobilized lipase is preferred given the limited stability of free lipase. Although research on flavonoid acylation for structural modification is actively being conducted, there is a need to explore continuous production for enhancing the industrial application of flavonoid esters. Therefore, in this study, we aimed to produce puerarin palmitate innovatively by selecting an effective lipase, developing an immobilized lipase, characterizing the enzymatic reaction, and designing a semi-continuous reactor system. Results Lipase from Thermomyces lanuginosus (TL) was found to be the most suitable for the synthesis of puerarin palmitate; it was successfully immobilized on glutaraldehyde-activated silica gel (GASG). TL GASG showed greater solvent, thermal, and operational stability than those of commercial immobilized lipases. Furthermore, TL GASG use resulted in a puerarin palmitate conversion rate of 97.17% within 3 h. Additionally, the reusability of TL GASG for this reaction was higher than that of commercially available immobilized lipases. Upon using a semi-continuous reactor, the cumulative conversion rate of puerarin palmitate remained above 85% after more than ten substrate injections. Conclusions The results support the feasibility of the continuous production of flavonoid esters, underscoring their diverse industrial applications. A biorefinery strategy is anticipated to be proposed to utilize P. lobata extract biomass for flavonoid ester production. Moreover, optimizing continuous reactors through statistical experimental design is expected to enhance flavonoid ester production efficiency; these aspects will be explored in future research. Graphical Abstract