Author:
Wang Siran,Hu Shiwei,Dong Zhihao,Li Junfeng,Zhao Jie,Nazar Mudasir,Kaka Niaz Ali,Shao Tao
Abstract
Abstract
Background
The purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.
Results
MZMZ had higher (P < 0.05) lactic acid contents, and lower (P < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of Lactobacillus in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (P < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (P < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.
Conclusions
The silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, Hafnia-Obesumbacterium, hetero-fermentative and acid-resistant Lactobacillus took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.
Graphical Abstract
Funder
Joint Fund for Regional Innovation and Development of National Natural Science Foundation of China
National Natural Science Foundation of China
Major Science and Technology Special Project of Tibet-Technological Mode Innovation and Demonstration of Spatio-temporal Expansion of Tibet Prataculture and Key Laboratory of Forage
Publisher
Springer Science and Business Media LLC
Subject
Agronomy and Crop Science,Biochemistry,Food Science,Biotechnology